Prereq.:BIOL 2153 and CHEM 2262 or CHEM 2462. BIOL 4087 or BIOL 4093 recommended. Theory and application of physical techniques to the study of biological macromolecules; spectroscopy (UV-VIS absorption and fluorescence, circular dichroism, IR, NMR, X-ray diffraction); helix-coil theory; theories of ligand binding.